Journal: Molecular Medicine Reports

Article Title: High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma

doi: 10.3892/mmr.2016.6033

Figure Lengend Snippet: Expression of B23 in bladder urothelial carcinoma and adjacent non-cancerous tissues, and normal human bladder and bladder cancer cells. (A) B23 expression in carcinoma and adjacent non-cancerous tissues was detected by immunohistochemical staining. (B) The percentage of B23-positive cases in 95 tumor tissues and the matched adjacent tissues was detected by immunohistochemistry analysis. There was a statistically significant increase in B23 staining between bladder urothelial carcinoma tissues and adjacent tissues. **P<0.01. (C) Protein and (D) mRNA expression levels of B23 in normal bladder tissues and tumors from bladder urothelial carcinoma patients were detected via western blotting and RT-qPCR, respectively. Protein and mRNA expression levels of B23 were increased in carcinoma tissues compared with adjacent tissues. (E) Protein and (F) mRNA expression levels of B23 in SV-HUC-1 normal human bladder cells and RT-4, BI-87, 253 J, J82 and T24 bladder cancer cell lines were detected by western blotting and RT-qPCR, respectively. B23 was highly expressed in bladder cancer cells. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; N, normal bladder tissues; T, tumor tissues.

Article Snippet: Non-specific antigen-antibody reactions were inhibited using an immunohistochemistry Protein Blocker-serum and Azide Free (MB-071-0100, Rockland Immunochemicals, Inc., Pottstown, PA, USA) for 5 min, following which the slides were washed thoroughly with PBS.

Techniques: Expressing, Immunohistochemical staining, Staining, Immunohistochemistry, Western Blot, Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction

Journal: Respiratory Research

Article Title: Following damage, the majority of bone marrow-derived airway cells express an epithelial marker

doi: 10.1186/1465-9921-7-145

Figure Lengend Snippet: Widefield visualisation of Y positive donor-derived cells and expression of CD45 and cytokeratin fall into four classes . CD45 positive stained cells (CY3-red) were identified on tracheal sections, photographed, and their positions recorded. The CD45 signal was lost in subsequent manipulations. Sections were then subjected to Y chromosome FISH (Tx-Red) and immunohistochemistry for cytokeratin (FITC-green). Donor-derived (Y FISH positive) cells fell into four classes (Table 2). Figure 1A shows a CD45 positive (i) and cytokeratin positive (ii) coincident cell arrowed; CD45 positive (iii) and cytokeratin negative (iv) coincident cell arrowed; CD45 negative (not shown) and cytokeratin positive (v,vi,vii,viii); CD45 negative (not shown) and cytokeratin negative (ix, x) arrowed cells. ix also contains a cytokeratin positive cell marked with an arrowhead. 1B top panel shows two CD45 positive (i) and cytokeratin positive (ii) cells and the brightfield staining of this tracheal section (iii) with cells marked with arrows; Figure 1B bottom panel shows a CD45 positive (iv), cytokeratin negative (v) cell and photomicrograph (vi) shows brightfield staining of the same region with the cell marked with arrowhead. shows cytokeratin staining alone of tracheal sections and also cytokeratin staining after Y chromosome FISH illustrating that the distribution of cytokeratin staining is the same. Scale bar 5 uM.

Article Snippet: Spleen was used as a positive control for CD45 as detailed on IHC World online information center for immunohistochemistry [ ].

Techniques: Derivative Assay, Expressing, Staining, Immunohistochemistry

Journal: Respiratory Research

Article Title: Following damage, the majority of bone marrow-derived airway cells express an epithelial marker

doi: 10.1186/1465-9921-7-145

Figure Lengend Snippet: Deconvolved visualisation of cells with Y chromosome FISH and cytokeratin immunohistochemistry . Examples of Y chromosome positive cells by Y FISH (Tx-Red) and cytokeratin immunohistochemistry (FITC-green). Arrows mark cells positive for Y chromosome and either positive for cytokeratin (A,B,C,D) or negative (E and F). Videos of positive and negative cells can be found in additional files (cytokeratin positive movie) and (cytokeratin negative movie).

Article Snippet: Spleen was used as a positive control for CD45 as detailed on IHC World online information center for immunohistochemistry [ ].

Techniques: Immunohistochemistry

Journal: Respiratory Research

Article Title: Following damage, the majority of bone marrow-derived airway cells express an epithelial marker

doi: 10.1186/1465-9921-7-145

Figure Lengend Snippet: Co-localisation of Y FISH and cytokeratin immunosignal in single cells . Female animals engrafted with male bone marrow were sacrificed after three months and cells dissociated from the tracheas were cytospun onto slides and subjected to Y FISH (red) and cytokeratin immunocytochemistry (green). Y positive (donor derived) cells are arrowed and both Y chromosome positive and cytokeratin negative (panels A,G and I) and Y positive, cytokeratin positive cells (panel B,C and E) were observed. Non-arrowed cells in panels A and B are cytokeratin positive but not Y FISH positive and presumably host female derived. Panels C and G show donor cells (arrow) stained for presence of Y chromosome and then the slides were counterstained with H and E (panel D and H respectively). Panels E and I show the donor Y FISH positive cells (arrowed) with panels F and J showing the same cells stained with H and E. The donor cell (arrowed) in panels I and J is cytokeratin negative and has macrophage morphology.

Article Snippet: Spleen was used as a positive control for CD45 as detailed on IHC World online information center for immunohistochemistry [ ].

Techniques: Immunocytochemistry, Derivative Assay, Staining

Journal: Frontiers in Immunology

Article Title: Multi-omic analysis of the tumor microenvironment shows clinical correlations in Ph1 study of atezolizumab +/- SoC in MM

doi: 10.3389/fimmu.2023.1085893

Figure Lengend Snippet: Unsupervised integrative clustering analysis at baseline schema (A) using (B) CyTOF, (C) Olink, (D) microenvironment-specific (CD138-negative), (E) tumor-specific (CD138-positive), and (F) SNF integrated data. Cohort A: Atezo monotherapy; B1: Atezo+Len; C: Atezo+Len; D1/D2/D3: Atezo+Dara; E1: Atezo+Dara+Len; F1/F2: Atezo+Dara+Pom; F3: Dara+Pom+Dex SNF, similarity network fusion; T, tumor; TME, tumor microenvironment. The heatmaps show clustered similarity matrices, with higher values depicted with darker shades indicating greater similarity between patients. In addition, the number of patients per cohort are color coded in the tables beneath each heatmap.

Article Snippet: CD8+ T-cell activation and proliferation were analyzed using %CD8+HLA-DR+Ki-67+.23 Formalin-fixed and decalcified paraffin-embedded tissue sections (4 mm thickness) taken at baseline were used for dual-plex immunohistochemistry (IHC) assay (CD138/tartrate-resistant acid phosphatase [TRAP]) at Histogenics Corp (MA, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Multi-omic analysis of the tumor microenvironment shows clinical correlations in Ph1 study of atezolizumab +/- SoC in MM

doi: 10.3389/fimmu.2023.1085893

Figure Lengend Snippet: Integrative analysis of on-treatment samples schema (A) using (B) CyTOF, (C) microenvironment-specific (CD138-negative), (D) tumor-specific (CD138-positive), and (E) SNF data. Cohort A: Atezo monotherapy; B1: Atezo+Len; C: Atezo+Len; D1/D2/D3: Atezo+Dara; E1: Atezo+Dara+Len; F1/F2: Atezo+Dara+Pom; F3: Dara+Pom+Dex. Atezo, atezolizumab; C, cycle; Combo, combination; D, day; Dara, daratumumab; Mono, monotherapy; SNF, similarity network fusion. The heatmaps show clustered similarity matrices, with higher values depicted with darker shades indicating greater similarity between patients.

Article Snippet: CD8+ T-cell activation and proliferation were analyzed using %CD8+HLA-DR+Ki-67+.23 Formalin-fixed and decalcified paraffin-embedded tissue sections (4 mm thickness) taken at baseline were used for dual-plex immunohistochemistry (IHC) assay (CD138/tartrate-resistant acid phosphatase [TRAP]) at Histogenics Corp (MA, USA).

Techniques:

Journal: Frontiers in Immunology

Article Title: Multi-omic analysis of the tumor microenvironment shows clinical correlations in Ph1 study of atezolizumab +/- SoC in MM

doi: 10.3389/fimmu.2023.1085893

Figure Lengend Snippet: Pairwise longitudinal analysis of (A) CyTOF, (B) microenvironment-specific (CD138-negative), and (C) tumor-specific (CD138-positive) data, and comparison of cell frequency in responders and non-responders of (D) CD8 TEMRA cells, (E) CD4 TEMRA cells, (F) DN T cells and (G) Tregs, and their distribution in each cluster. Mann-Whitney and Tukey’s multiple comparisons tests, *P<0.05; **P<0.01; *** P<0.001. Atezo, atezolizumab; CL, cluster; Dara, daratumumab; DN, double-negative; Mono, monotherapy; NK, natural killer; NR, non-responder; PD, programmed death; PDL, programmed death ligand; R, responder; Refract., refractory; TEMRA, terminally differentiated effector memory T cell; Treg, regulatory T cell.

Article Snippet: CD8+ T-cell activation and proliferation were analyzed using %CD8+HLA-DR+Ki-67+.23 Formalin-fixed and decalcified paraffin-embedded tissue sections (4 mm thickness) taken at baseline were used for dual-plex immunohistochemistry (IHC) assay (CD138/tartrate-resistant acid phosphatase [TRAP]) at Histogenics Corp (MA, USA).

Techniques: MANN-WHITNEY

Journal: Frontiers in Immunology

Article Title: Multi-omic analysis of the tumor microenvironment shows clinical correlations in Ph1 study of atezolizumab +/- SoC in MM

doi: 10.3389/fimmu.2023.1085893

Figure Lengend Snippet: Post-treatment one vs all gene set analysis using (A) microenvironment-specific (CD138-negative), and (B) tumor-specific (CD138-positive) data. mDC, myeloid dendritic cells; NK, natural killer.

Article Snippet: CD8+ T-cell activation and proliferation were analyzed using %CD8+HLA-DR+Ki-67+.23 Formalin-fixed and decalcified paraffin-embedded tissue sections (4 mm thickness) taken at baseline were used for dual-plex immunohistochemistry (IHC) assay (CD138/tartrate-resistant acid phosphatase [TRAP]) at Histogenics Corp (MA, USA).

Techniques: